Journal: EXCLI Journal

Article Title: The regulatory role and mechanism of TRPV3 on apoptosis and inflammation in osteoarthritis

doi: 10.17179/excli2024-8109

Figure Lengend Snippet: Expression of TRPV3 in IL-1β treated C28/I2 chondrocyte cells. (A) expression of TRPV3 in mRNA level was assessed with qRT-PCR in the C28/I2 cells treated with IL-1β, Si-TRPV3+IL-1β, or Si-NC +IL-1β (siRNA negative control+IL-1β) as siRNA negative control, or no treatment as control. (B) expression of TRPV3 in protein level was evaluated by western blot. (C) Selected blots reflect corresponding protein levels. Data are shown as mean ± SD. All experiments were evaluated three times. Significance: ns > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control and # p < 0.05, ## p < 0.01, ### p < 0.001 and, #### p < 0.0001 vs. IL-1β

Article Snippet: Metabion, Germany, generated the small interfering RNA (siRNA) and controls against TRPV3.

Techniques: Expressing, Quantitative RT-PCR, Negative Control, Control, Western Blot

Journal: EXCLI Journal

Article Title: The regulatory role and mechanism of TRPV3 on apoptosis and inflammation in osteoarthritis

doi: 10.17179/excli2024-8109

Figure Lengend Snippet: Examination of cellular apoptosis. The impact of Si-TRPV3 on apoptosis of IL-1β-treated C28/I2 chondrocyte cells was assessed using flow cytometry. (A) Control group; (B) C28/I2 cells were treated with 10 ng/mL of IL-1β; (C) cells received Si-TRPV3 treatment for 6 hours, followed by the addition of IL-1β (10 ng/mL) for 24 hours; (D) cells were treated with Si-NC in conjunction with IL-1β, (E) A graph illustrating the average percentage of early and late apoptosis for each treatment in the apoptosis assay. (F) Cell viability was evaluated with the MTT test. The error bars represent the standard deviation (SD) of three independent investigations. Significance: ns > 0.05, *p < 0.05, **p < 0.01 and ****p < 0.0001 vs. Control group and # p < 0.05, ## p < 0.01 and #### p < 0.0001 vs. IL-1β group

Article Snippet: Metabion, Germany, generated the small interfering RNA (siRNA) and controls against TRPV3.

Techniques: Flow Cytometry, Control, Apoptosis Assay, Standard Deviation

Journal: EXCLI Journal

Article Title: The regulatory role and mechanism of TRPV3 on apoptosis and inflammation in osteoarthritis

doi: 10.17179/excli2024-8109

Figure Lengend Snippet: Cell cycle phase distribution analysis in control and treated C28/I2 chondrocyte cells. (A) Distribution of cell cycle phases in control cells. (B) Following treatment with a 10 ng/ml concentration of IL-1β, a significant increase in the number of cells in the sub-G1 phase is observed, indicating the presence of apoptotic cells. (C) After 24-hour treatment with 10 ng/ml Si-TRPV3+IL-1β, the number of apoptotic cells was significantly decreased compared with the IL-1β-only group. (D) After 24-hour treatment with 10 ng/ml Si-NC+IL-1β, the count of apoptotic cells was not significantly different from that of the IL-1β-only group. (E) Shows findings from three independent tests for the distribution of cell cycle phases. Error bars show the SD (standard deviation) of three separate experiments. Significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. control and # p < 0.05, ## p < 0.01, ### p < 0.001 and #### p < 0.0001 vs. IL-1β.

Article Snippet: Metabion, Germany, generated the small interfering RNA (siRNA) and controls against TRPV3.

Techniques: Control, Concentration Assay, Standard Deviation

Journal: EXCLI Journal

Article Title: The regulatory role and mechanism of TRPV3 on apoptosis and inflammation in osteoarthritis

doi: 10.17179/excli2024-8109

Figure Lengend Snippet: The effect of Si-TRPV3 on the mRNA and protein expression of apoptosis genes. The mRNA expression levels of (A) Caspase-3, (B) Bcl-2, and (C) Bax were evaluated using qRT-PCR. Protein levels of (D) pro-Caspase-3, (E) Cleaved Caspase-3, (F) Bcl-2, and (G) Bax were assessed using western blot analysis. (H) The selected blots indicate the levels of the corresponding proteins. Data are shown as mean ± SD. All experiments were examined three times. Significance: ns > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control and ns > 0.05, # p < 0.05, ## p < 0.01 and, ### p < 0.001 vs. IL-1β

Article Snippet: Metabion, Germany, generated the small interfering RNA (siRNA) and controls against TRPV3.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: EXCLI Journal

Article Title: The regulatory role and mechanism of TRPV3 on apoptosis and inflammation in osteoarthritis

doi: 10.17179/excli2024-8109

Figure Lengend Snippet: Effects of TRPV3 knockdown on the expression of inflammation genes. The cells underwent pretreatment with Si-TRPV3 for 6 hours before a subsequent treatment with 10 ng/ml IL-1β for 24 hours. The mRNA expression levels of (A) iNOS and (B) COX-2 were assessed by qRT-PCR, and the protein expression levels of (C) iNOS and (D) COX-2 were measured by western blot, respectively. (E) Selected blots reflect corresponding protein levels. Data are shown as mean ± SD. All experiments were examined three times. Significance: ns > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. control and ns > 0.05, # p < 0.05, ## p < 0.01, ### p < 0.001 and #### p < 0.0001 vs. IL-1β

Article Snippet: Metabion, Germany, generated the small interfering RNA (siRNA) and controls against TRPV3.

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Control